PhD Defense · UMCG · Groningen

From Vulnerability to Resilience

Molecular Mechanisms of Cancer Therapy-Induced Cardiotoxicity
🗓 May 21, 2026 · 12:45 · Academie Gebouw — arrive 12:15
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Core Narrative — Practice Daily (90 seconds)

"We started with a clinical paradox: some patients develop severe heart failure after low doses of anthracyclines, while others tolerate much higher cumulative doses with no cardiac injury. We hypothesized this reflects constitutional vulnerability — not treatment effects. Using patient-derived iPSC cardiomyocytes and 3D engineered heart tissues, we found pre-existing mitochondrial genome instability (~77% insertion-biased mutations in TOX vs ~54% in RES) and inadequate antioxidant defenses (GPX1↓, GSTM1↓), creating a constitutionally vulnerable cardiac phenotype. This shifts the paradigm: not 'how much drug causes heart failure' but 'who is already positioned close to cardiac failure thresholds.' Clinical implication: biomarker-guided personalized cardioprotection before the first dose."

22-Day Study Plan

April 28 – May 19 · 4 hrs/day · ADHD-optimized: 2 × 2-hour blocks

🧠 ADHD Protocol: Morning 09:00–11:00 · Afternoon 14:00–16:00 · Hard stop at 4h · Quiz after each block · Each task ≈ 30–45 min.

Active Recall Quiz

Spaced repetition · Stop when 90% correct · Progress saved to D1

50 Essential Papers

Track reading · Quiz per paper · Prioritized by defense relevance

2022 ESC Cardio-Oncology Guidelines

Lyon AR et al. Eur Heart J 2022 · Risk stratification · Drug-specific protocols · Clinical thresholds

Baseline CV Risk Categories (HFA-ICOS)

CategoryLVEFKey CriteriaThesis Context
Low≥55%No CV risk factors, no CV diseaseRES phenotype — buffer intact
Moderate≥50%≥2 uncontrolled CV risk factors OR 1 CV diseaseIntermediate risk
High40–49%Prior cardiotoxicity, severe valve disease, genetic CMTOX phenotype risk zone
Very High<40%Decompensated HF, recent ACS, serious arrhythmiaConstitutional threshold crossed

CTRCD Definition

New LVEF drop >10 percentage points to below 53%, with or without symptoms — OR — any new LVEF drop to <53% with new symptoms of HF

Subclinical CTRCD

GLS relative reduction >15% from baseline AND/OR new troponin elevation (above URL). LVEF still preserved.

Thesis Link

TOX patients cross this threshold at lower doses. mtDNA biomarker predicts this before GLS drops.

Drug-Specific Monitoring

DrugBaselineDuringAfter
Anthracyclines HighEcho+GLS, hsTnI, ECGEcho q3 cycles; hsTnI each cycle (high risk)3mo, 6mo, 12mo, annual ×5yr
TrastuzumabEcho, hsTnI, ECGEcho q3 monthsEcho 6mo + 12mo
CDK4/6 inhibitorsECG (QTc <450ms!), electrolytesECG day 14 cycle 1, day 1 cycle 2, monthly ×3Echo if symptoms
ICIsTroponin, ECG, echoTroponin + ECG each cycle (high risk)Annual echo ×5yr
VEGF inhibitorsBP every visitBP monitoring; stop if grade 3 HTNBP ×1yr

Cardioprotection

ACEi + Carvedilol

Most studied. Start when LVEF 50–54% or GLS >15% relative reduction. Continue ≥6 months.

Dexrazoxane

Iron chelator. Recommended at >300 mg/m² doxorubicin equivalent. Reduces cardiotoxicity ~60–70%.

SGLT2 Inhibitors

Emerging evidence. Active trials. Thesis relevance: metabolic protection of failing cardiomyocytes.

ICI Myocarditis — Immediate Protocol

Incidence 0.5–1.5% · Case fatality up to 50% if fulminant · Every hour matters

StepActionWhy
1Withhold ICI immediatelyStop ongoing immune damage
2Methylprednisolone 1g/day IV ×3–5 daysImmunosuppression — delay = mortality
3Cardiac MRI (T2 edema + LGE)Confirms diagnosis, quantifies inflammation
4Biopsy if uncertain (CD3/CD8+ infiltrate)Definitive diagnosis
5Refractory: mycophenolate / abataceptSecond-line immunosuppression

Test Your Guidelines Knowledge

15 questions · Risk categories, CTRCD definition, drug protocols, ICI myocarditis

Opposition Committee

Defense order · May 21, 2026 · Know their lens, their papers, their questions

The Disarming Move: Reference each member's paper by name ("Your 2022 Nature paper on cGAS/STING…"). One sentence transforms examination into conversation.

Defense Strategy

The Three Laws

1

Acknowledge Before Defending

"That is an important limitation. We addressed it by…" — Never be defensive. Shows scientific maturity.

2

Data Before Opinion

Ground every answer in your actual figures before making interpretive claims.

3

Offer the Future

For every limitation, propose the specific experiment that would resolve it.

Know These Cold

Quick Reference Per Committee Member

Chapter Map

🧠 4-Hour Daily Goal Reached! Stop here — sleep consolidates memory better than more studying. 🌙